Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: Chemical structure of Fasudil hydrochloride.

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques:

Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: Ex vivo permeation study of Fasudil from Fasudil simple solution through excised bovine cornea and sclera for 6 h. Results are expressed (n = 3 ± SD), t -test was performed.

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques: Ex Vivo

Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: Flux, apparent permeability coefficient (Papp), lag time (tL), and regression coefficient (R 2 ) of Fasudil simple solution through cornea and sclera.

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques: Permeability

Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: Scanning electron micrographs of optimized Cs nanoparticles loaded with Fasudil hydrochloride.

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques:

Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: Cumulative release of Fasudil loaded into Cs NPs and Fasudil solution (control) in simulated tear fluid (STF). Results are presented as mean ± SD, n = 3.

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques: Control

Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: The coefficients of determination (R 2 ) and the release exponent (n) were obtained through model fitting of the release data of Fasudil from the optimized Cs NPs (results represent mean values ± SD, n = 3).

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques:

Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: Trans-corneal permeation profiles of Fasudil from Cs NPs and Fasudil solution (mean ± SD, n = 3).

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques:

Journal: Pharmaceutics

Article Title: Exploring the Ocular Absorption Pathway of Fasudil Hydrochloride towards Developing a Nanoparticulate Formulation with Improved Performance

doi: 10.3390/pharmaceutics16010112

Figure Lengend Snippet: Flux, apparent permeability coefficient (Papp), lag time (tL), and regression coefficient (R 2 ) of Fasudil through cornea from Fasudil simple solution and Fasudil-Cs NPs.

Article Snippet: Fasudil hydrochloride was obtained from Biosynth Ltd., Compton, UK.

Techniques: Permeability

Journal: Experimental and Therapeutic Medicine

Article Title: The effect of the Ras homolog gene family (Rho), member A/Rho associated coiled-coil forming protein kinase pathway in atrial fibrosis of type 2 diabetes in rats

doi: 10.3892/etm.2014.1843

Figure Lengend Snippet: The effects of fasudil on glucose metabolism parameters in rats at week 24.

Article Snippet: Diabetic rats were randomly divided into two groups: i) Treated diabetic rats were maintained on a HFD and received fasudil hydrochloride hydrate (10 mg/kg/day; i.p.) (Tianjin Hongri Company, Tianjin, China) every day for 14 weeks; and ii) untreated diabetic rats were treated with i.p. injection of sterile vehicle every day for 14 weeks.

Techniques: Control

Journal: Experimental and Therapeutic Medicine

Article Title: The effect of the Ras homolog gene family (Rho), member A/Rho associated coiled-coil forming protein kinase pathway in atrial fibrosis of type 2 diabetes in rats

doi: 10.3892/etm.2014.1843

Figure Lengend Snippet: The deposition of collagen in the atrial interstitium in Masson-stained sections of the control, diabetic and diabetic rats treated with fasudil (DM+fas). Heart tissues from the diabetic group show focal regions of fibrosis (blue) in the interstitium (P<0.01 vs. the control and DM+fas groups). Magnification, ×400. The number of rats in each group was six.

Article Snippet: Diabetic rats were randomly divided into two groups: i) Treated diabetic rats were maintained on a HFD and received fasudil hydrochloride hydrate (10 mg/kg/day; i.p.) (Tianjin Hongri Company, Tianjin, China) every day for 14 weeks; and ii) untreated diabetic rats were treated with i.p. injection of sterile vehicle every day for 14 weeks.

Techniques: Staining, Control

Journal: Experimental and Therapeutic Medicine

Article Title: The effect of the Ras homolog gene family (Rho), member A/Rho associated coiled-coil forming protein kinase pathway in atrial fibrosis of type 2 diabetes in rats

doi: 10.3892/etm.2014.1843

Figure Lengend Snippet: mRNA expression levels of (A) RhoA, (B) ROCK1 and (C) ROCK2, in untreated diabetic, control and DM+fas rats are evaluated by quantitative polymerase chain reaction. The intensities of RhoA, ROCK1 and ROCK2 were standardized to that of GAPDH. The number of rats in each group was six. The mRNA expression levels of RhoA, ROCK1 and ROCK2 were significantly increased in diabetic rats and decreased in DM+fas rats. * P<0.01 vs. control rats, ** P<0.01 vs. diabetic rats. DM+fas, diabetic treated with fasudil.

Article Snippet: Diabetic rats were randomly divided into two groups: i) Treated diabetic rats were maintained on a HFD and received fasudil hydrochloride hydrate (10 mg/kg/day; i.p.) (Tianjin Hongri Company, Tianjin, China) every day for 14 weeks; and ii) untreated diabetic rats were treated with i.p. injection of sterile vehicle every day for 14 weeks.

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: The effect of the Ras homolog gene family (Rho), member A/Rho associated coiled-coil forming protein kinase pathway in atrial fibrosis of type 2 diabetes in rats

doi: 10.3892/etm.2014.1843

Figure Lengend Snippet: The mRNA expression levels of (A) type I and (B) type III procollagen in untreated diabetic, control and DM+fas rats was evaluated by quantitative polymerase chain reaction. The intensities of type I and III procollagen were standardized to that of GAPDH. The number of rats in each group was six. The mRNA expression levels of type I and III procollagen were significantly increased in diabetic rats and decreased in DM+fas rats. * P<0.01 vs. control rats, ** P<0.01 vs. diabetic rats. DM+fas, diabetic treated with fasudil.

Article Snippet: Diabetic rats were randomly divided into two groups: i) Treated diabetic rats were maintained on a HFD and received fasudil hydrochloride hydrate (10 mg/kg/day; i.p.) (Tianjin Hongri Company, Tianjin, China) every day for 14 weeks; and ii) untreated diabetic rats were treated with i.p. injection of sterile vehicle every day for 14 weeks.

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: The effect of the Ras homolog gene family (Rho), member A/Rho associated coiled-coil forming protein kinase pathway in atrial fibrosis of type 2 diabetes in rats

doi: 10.3892/etm.2014.1843

Figure Lengend Snippet: The protein levels of (A) RhoA, (B) ROCK1 and (C) ROCK2 in control, diabetic and DM+fas rats were evaluated by western blot analyses. The protein levels of RhoA, ROCK1 and ROCK2 were significantly increased in diabetic rats and decreased in DM+fas rats. * P<0.05 vs. control rats, ** P<0.05 vs. diabetic rats. DM+fas, diabetic treated with fasudil.

Article Snippet: Diabetic rats were randomly divided into two groups: i) Treated diabetic rats were maintained on a HFD and received fasudil hydrochloride hydrate (10 mg/kg/day; i.p.) (Tianjin Hongri Company, Tianjin, China) every day for 14 weeks; and ii) untreated diabetic rats were treated with i.p. injection of sterile vehicle every day for 14 weeks.

Techniques: Control, Western Blot